Vertical transmission of HIV in Ghanaian women diagnosed in cord blood and post‐natal samples
Identifieur interne : 000E80 ( Main/Exploration ); précédent : 000E79; suivant : 000E81Vertical transmission of HIV in Ghanaian women diagnosed in cord blood and post‐natal samples
Auteurs : Lucia Fischetti [Royaume-Uni] ; Kwabena Danso [Ghana] ; Albert Dompreh [Ghana] ; Victor Addo [Ghana] ; Lars Haaheim [Norvège] ; Jean-Pierre Allain [Royaume-Uni]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2005-11.
Descripteurs français
- KwdFr :
- ARN viral (sang), Analyse de séquence d'ADN, Charge virale, Complications de la grossesse et maladies infectieuses (diagnostic), Complications de la grossesse et maladies infectieuses (virologie), Données de séquences moléculaires, Femelle, Ghana (épidémiologie), Grossesse, Humains, Infections à VIH (diagnostic), Infections à VIH (transmission), Infections à VIH (virologie), Nouveau-né, Phylogénie, Prévalence, Sang foetal (virologie), Transmission verticale de maladie infectieuse, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (isolement et purification), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (physiologie).
- MESH :
- diagnostic : Complications de la grossesse et maladies infectieuses, Infections à VIH.
- génétique : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- isolement et purification : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- physiologie : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- sang : ARN viral.
- virologie : Complications de la grossesse et maladies infectieuses, Infections à VIH, Sang foetal.
- épidémiologie : Ghana, Infections à VIH.
- Pascal (Inist)
- MESH :
- Wicri :
English descriptors
- KwdEn :
- Aids fischetti, Blood, Blood donors, Blood pairs, Clone, Cord, Cord blood, Cord blood clones, Cord blood quasispecies, Cord blood samples, Cord clones, Cord quasispecies, Detectable virus, Female, Fetal Blood (virology), Fischetti, Genetic distance, Genetic diversity, Genetic variability, Genital secretions, Genotype, Ghana, Ghana (epidemiology), HIV Infections (diagnosis), HIV Infections (transmission), HIV Infections (virology), HIV-1 (genetics), HIV-1 (isolation & purification), HIV-1 (physiology), Higher prevalence, Human, Human immunodeficiency virus, Human virus, Human virus type, Humans, Immune response, Infant, Newborn, Infection prevalence, Infectious Disease Transmission, Vertical, Intrapartum transmission, Komfo anokye teaching hospital, Maternal, Maternal clones, Maternal plasma, Maternal quasispecies, Maternal variants, Median, Minor variant, Molecular Sequence Data, Molecular epidemiology, Multiple variants, Newborn sample, Newborn samples, Novel diagnostics, Nucleotide distances, Other studies, Overall rate, Perinatal, Perinatal transmission, Peripheral blood, Phylogeny, Pregnancy, Pregnancy Complications, Infectious (diagnosis), Pregnancy Complications, Infectious (virology), Pregnant women, Prevalence, Quasispecies, RNA, Viral (blood), Second round, Selective processes, Sequence Analysis, DNA, Several studies, Small number, Study group, Subtype, Subtype distribution, Subtypes, Transfusion medicine, Transmission, Utero, Utero transmission, Variant, Vertical transmission, Viral, Viral Load, Viral compartmentalization, Viral load, Viral load distribution, Viral quasispecies, Virol, Virus populations, Virus type.
- MESH :
- chemical , blood : RNA, Viral.
- geographic , epidemiology : Ghana.
- diagnosis : HIV Infections, Pregnancy Complications, Infectious.
- genetics : HIV-1.
- isolation & purification : HIV-1.
- physiology : HIV-1.
- transmission : HIV Infections.
- virology : Fetal Blood, HIV Infections, Pregnancy Complications, Infectious.
- Teeft :
- Aids fischetti, Blood donors, Blood pairs, Clone, Cord, Cord blood, Cord blood clones, Cord blood quasispecies, Cord blood samples, Cord clones, Cord quasispecies, Detectable virus, Female, Fischetti, Genetic distance, Genetic diversity, Genetic variability, Genital secretions, Genotype, Higher prevalence, Human virus, Human virus type, Humans, Immune response, Infant, Newborn, Infection prevalence, Infectious Disease Transmission, Vertical, Intrapartum transmission, Komfo anokye teaching hospital, Maternal, Maternal clones, Maternal plasma, Maternal quasispecies, Maternal variants, Median, Minor variant, Molecular Sequence Data, Molecular epidemiology, Multiple variants, Newborn sample, Newborn samples, Novel diagnostics, Nucleotide distances, Other studies, Overall rate, Perinatal, Perinatal transmission, Peripheral blood, Phylogeny, Pregnancy, Pregnant women, Prevalence, Quasispecies, Second round, Selective processes, Sequence Analysis, DNA, Several studies, Small number, Study group, Subtype, Subtype distribution, Subtypes, Transfusion medicine, Transmission, Utero, Utero transmission, Variant, Vertical transmission, Viral, Viral Load, Viral compartmentalization, Viral load, Viral load distribution, Viral quasispecies, Virol, Virus populations, Virus type.
Abstract
HIV RNA detection in the newborn is the main diagnostic tool for vertical transmission. Most infections are thought to occur peri‐ or post‐natally, hence preventive antiviral therapy administered days before and during delivery. This study used cord blood for molecular diagnosis, examined viral load and HIV‐1 subtypes as determinants of transmission, and compared molecular variability of maternal, cord blood, and post‐natal quasispecies. Ninety‐seven seropositive mother‐cord blood paired plasmas from Ghana were tested for HIV RNA. Viral load was quantified and a subgroup of 45 random women samples was typed and subtyped. HIV‐1 from infected pairs was cloned, sequenced, and analyzed phylogenetically. The prevalence of HIV infection in pregnant women was 3.3%. 13/97 cord blood samples (13.5%) contained HIV RNA. No correlation between either viral load at labor (range 103–107) or HIV‐1 subtype and in utero transmission was found. In both transmitting and non‐transmitting mothers, 56% of HIV‐1 strains were CRF02_AG. In three pairs, maternal and cord blood quasispecies were closely related, suggesting late pregnancy or perinatal transmission, while in four pairs, genetic distances suggested transmission earlier during gestation. Maternal viral load and genotype did not correlate with HIV‐1 pre‐natal transmission. HIV infection during gestation appears relatively frequent. J. Med. Virol. 77:351–359, 2005. © 2005 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20463
Affiliations:
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Infections (transmission)</term><term>HIV Infections (virology)</term><term>HIV-1 (genetics)</term><term>HIV-1 (isolation & purification)</term><term>HIV-1 (physiology)</term><term>Higher prevalence</term><term>Human</term><term>Human immunodeficiency virus</term><term>Human virus</term><term>Human virus type</term><term>Humans</term><term>Immune response</term><term>Infant, Newborn</term><term>Infection prevalence</term><term>Infectious Disease Transmission, Vertical</term><term>Intrapartum transmission</term><term>Komfo anokye teaching hospital</term><term>Maternal</term><term>Maternal clones</term><term>Maternal plasma</term><term>Maternal quasispecies</term><term>Maternal variants</term><term>Median</term><term>Minor variant</term><term>Molecular Sequence Data</term><term>Molecular epidemiology</term><term>Multiple variants</term><term>Newborn sample</term><term>Newborn samples</term><term>Novel diagnostics</term><term>Nucleotide distances</term><term>Other studies</term><term>Overall rate</term><term>Perinatal</term><term>Perinatal transmission</term><term>Peripheral blood</term><term>Phylogeny</term><term>Pregnancy</term><term>Pregnancy Complications, Infectious (diagnosis)</term><term>Pregnancy Complications, Infectious (virology)</term><term>Pregnant women</term><term>Prevalence</term><term>Quasispecies</term><term>RNA, Viral (blood)</term><term>Second round</term><term>Selective processes</term><term>Sequence Analysis, DNA</term><term>Several studies</term><term>Small number</term><term>Study group</term><term>Subtype</term><term>Subtype distribution</term><term>Subtypes</term><term>Transfusion medicine</term><term>Transmission</term><term>Utero</term><term>Utero transmission</term><term>Variant</term><term>Vertical transmission</term><term>Viral</term><term>Viral Load</term><term>Viral compartmentalization</term><term>Viral load</term><term>Viral load distribution</term><term>Viral quasispecies</term><term>Virol</term><term>Virus populations</term><term>Virus type</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (sang)</term><term>Analyse de séquence d'ADN</term><term>Charge virale</term><term>Complications de la grossesse et maladies infectieuses (diagnostic)</term><term>Complications de la grossesse et maladies infectieuses (virologie)</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Ghana (épidémiologie)</term><term>Grossesse</term><term>Humains</term><term>Infections à VIH (diagnostic)</term><term>Infections à VIH (transmission)</term><term>Infections à VIH (virologie)</term><term>Nouveau-né</term><term>Phylogénie</term><term>Prévalence</term><term>Sang foetal (virologie)</term><term>Transmission verticale de maladie infectieuse</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (isolement et purification)</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Ghana</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>HIV Infections</term><term>Pregnancy Complications, Infectious</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Complications de la grossesse et maladies infectieuses</term><term>Infections à VIH</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>HIV Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Complications de la grossesse et maladies infectieuses</term><term>Infections à VIH</term><term>Sang foetal</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Fetal Blood</term><term>HIV Infections</term><term>Pregnancy Complications, Infectious</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Ghana</term><term>Infections à VIH</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Charge virale</term><term>Femelle</term><term>Ghana</term><term>Homme</term><term>Sang</term><term>Soustype</term><term>Transmission verticale</term><term>Virus immunodéficience humaine</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Aids fischetti</term><term>Blood donors</term><term>Blood pairs</term><term>Clone</term><term>Cord</term><term>Cord blood</term><term>Cord blood clones</term><term>Cord blood quasispecies</term><term>Cord blood samples</term><term>Cord clones</term><term>Cord quasispecies</term><term>Detectable virus</term><term>Female</term><term>Fischetti</term><term>Genetic distance</term><term>Genetic diversity</term><term>Genetic variability</term><term>Genital secretions</term><term>Genotype</term><term>Higher prevalence</term><term>Human virus</term><term>Human virus type</term><term>Humans</term><term>Immune response</term><term>Infant, Newborn</term><term>Infection prevalence</term><term>Infectious Disease Transmission, Vertical</term><term>Intrapartum transmission</term><term>Komfo anokye teaching hospital</term><term>Maternal</term><term>Maternal clones</term><term>Maternal plasma</term><term>Maternal quasispecies</term><term>Maternal variants</term><term>Median</term><term>Minor variant</term><term>Molecular Sequence Data</term><term>Molecular epidemiology</term><term>Multiple variants</term><term>Newborn sample</term><term>Newborn samples</term><term>Novel diagnostics</term><term>Nucleotide distances</term><term>Other studies</term><term>Overall rate</term><term>Perinatal</term><term>Perinatal transmission</term><term>Peripheral blood</term><term>Phylogeny</term><term>Pregnancy</term><term>Pregnant women</term><term>Prevalence</term><term>Quasispecies</term><term>Second round</term><term>Selective processes</term><term>Sequence Analysis, DNA</term><term>Several studies</term><term>Small number</term><term>Study group</term><term>Subtype</term><term>Subtype distribution</term><term>Subtypes</term><term>Transfusion medicine</term><term>Transmission</term><term>Utero</term><term>Utero transmission</term><term>Variant</term><term>Vertical transmission</term><term>Viral</term><term>Viral Load</term><term>Viral compartmentalization</term><term>Viral load</term><term>Viral load distribution</term><term>Viral quasispecies</term><term>Virol</term><term>Virus populations</term><term>Virus type</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Charge virale</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Grossesse</term><term>Humains</term><term>Nouveau-né</term><term>Phylogénie</term><term>Prévalence</term><term>Transmission verticale de maladie infectieuse</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">HIV RNA detection in the newborn is the main diagnostic tool for vertical transmission. Most infections are thought to occur peri‐ or post‐natally, hence preventive antiviral therapy administered days before and during delivery. This study used cord blood for molecular diagnosis, examined viral load and HIV‐1 subtypes as determinants of transmission, and compared molecular variability of maternal, cord blood, and post‐natal quasispecies. Ninety‐seven seropositive mother‐cord blood paired plasmas from Ghana were tested for HIV RNA. Viral load was quantified and a subgroup of 45 random women samples was typed and subtyped. HIV‐1 from infected pairs was cloned, sequenced, and analyzed phylogenetically. The prevalence of HIV infection in pregnant women was 3.3%. 13/97 cord blood samples (13.5%) contained HIV RNA. No correlation between either viral load at labor (range 103–107) or HIV‐1 subtype and in utero transmission was found. In both transmitting and non‐transmitting mothers, 56% of HIV‐1 strains were CRF02_AG. In three pairs, maternal and cord blood quasispecies were closely related, suggesting late pregnancy or perinatal transmission, while in four pairs, genetic distances suggested transmission earlier during gestation. Maternal viral load and genotype did not correlate with HIV‐1 pre‐natal transmission. HIV infection during gestation appears relatively frequent. J. Med. Virol. 77:351–359, 2005. © 2005 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Ghana</li><li>Norvège</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Angleterre de l'Est</li></region><settlement><li>Cambridge</li></settlement><orgName><li>Université de Cambridge</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Fischetti, Lucia" sort="Fischetti, Lucia" uniqKey="Fischetti L" first="Lucia" last="Fischetti">Lucia Fischetti</name></region><name sortKey="Allain, Jean Ierre" sort="Allain, Jean Ierre" uniqKey="Allain J" first="Jean-Pierre" last="Allain">Jean-Pierre Allain</name><name sortKey="Allain, Jean Ierre" sort="Allain, Jean Ierre" uniqKey="Allain J" first="Jean-Pierre" last="Allain">Jean-Pierre Allain</name></country><country name="Ghana"><noRegion><name sortKey="Danso, Kwabena" sort="Danso, Kwabena" uniqKey="Danso K" first="Kwabena" last="Danso">Kwabena Danso</name></noRegion><name sortKey="Addo, Victor" sort="Addo, Victor" uniqKey="Addo V" first="Victor" last="Addo">Victor Addo</name><name sortKey="Dompreh, Albert" sort="Dompreh, Albert" uniqKey="Dompreh A" first="Albert" last="Dompreh">Albert Dompreh</name></country><country name="Norvège"><noRegion><name sortKey="Haaheim, Lars" sort="Haaheim, Lars" uniqKey="Haaheim L" first="Lars" last="Haaheim">Lars Haaheim</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|étape= Exploration
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|clé= ISTEX:A1B968CCD0DD7DCBA22EA04C3533EB8185C35061
|texte= Vertical transmission of HIV in Ghanaian women diagnosed in cord blood and post‐natal samples
}}
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